The muscle proteins

The total amount of muscle proteins in mammals, including humans, exceeds that of any other protein. About 40 percent of the body weight of a healthy human adult weighing about 70 kilograms (150 pounds) is muscle, which is composed of about 20 percent muscle protein. Thus, the human body contains about 5 to 6 kilograms (11 to 13 pounds) of muscle protein. An albumin-like fraction of these proteins, originally called myogen, contains various enzymes—phosphorylase, aldolase, glyceraldehyde phosphate dehydrogenase, and others; it does not seem to be involved in contraction. The globulin fraction contains myosin, the contractile protein, which also occurs in blood platelets, small bodies found in blood. Similar contractile substances occur in other contractile structures; for example, in the cilia or flagella (whiplike organs of locomotion) of bacteria and protozoans. In contrast to the scleroproteins, the contractile proteins are soluble in salt solutions and susceptible to enzymatic digestion.

The energy required for muscle contraction is provided by the oxidation of carbohydrates or lipids. The term mechanochemical reaction has been used for this conversion of chemical into mechanical energy. The molecular process underlying the reaction is known to involve the fibrous muscle proteins, the peptide chains of which undergo a change in conformation during contraction.

Myosin, which can be removed from fresh muscle by adding it to a chilled solution of dilute potassium chloride and sodium bicarbonate, is insoluble in water. Myosin, solutions of which are highly viscous, consists of an elongated—probably double-stranded—peptide chain, which is coiled at both ends in such a way that a terminal globule is formed. The length of the molecule is approximately 160 nanometres and its average diameter 2.6 nanometres. The equivalent weight of each of the two terminal globules is approximately 30,000; the molecular weight of myosin is close to 500,000. Trypsin splits myosin into large fragments called meromyosin. Myosin contains many amino acids with positively and negatively charged side chains; they form 18 and 16 percent, respectively, of the total number of amino acids. Myosin catalyzes the hydrolytic cleavage of ATP (adenosine triphosphate). A smaller protein with properties similar to those of myosin is tropomyosin. It has a molecular weight of 70,000 and dimensions of 45 by 2 nanometres. More than 90 percent of its peptide chains are present in the α-helix form.

Myosin combines easily with another muscle protein called actin, the molecular weight of which is about 50,000; it forms 12 to 15 percent of the muscle proteins. Actin can exist in two forms—one, G-actin, is globular; the other, F-actin, is fibrous. Actomyosin is a complex molecule formed by one molecule of myosin and one or two molecules of actin. In muscle, actin and myosin filaments are oriented parallel to each other and to the long axis of the muscle. The actin filaments are linked to each other lengthwise by fine threads called S filaments. During contraction the S filaments shorten, so that the actin filaments slide toward each other, past the myosin filaments, thus causing a shortening of the muscle (for a detailed description of the process, see muscle: Striated muscle).