{"id":1864,"date":"2024-03-03T16:15:10","date_gmt":"2024-03-03T16:15:10","guid":{"rendered":"https:\/\/cake.appscodestudio.com\/?p=1864"},"modified":"2024-03-03T17:19:52","modified_gmt":"2024-03-03T17:19:52","slug":"nucleases","status":"publish","type":"post","link":"https:\/\/cake.appscodestudio.com\/index.php\/2024\/03\/03\/nucleases\/","title":{"rendered":"Nucleases"},"content":{"rendered":"\n<p>Nucleases are enzymes that hydrolytically&nbsp;cleave&nbsp;the phosphodiester backbone of DNA.&nbsp;Endonucleases cleave in the middle of chains, while exonucleases operate selectively by degrading from the end of the chain. Nucleases that act on both single- and double-stranded DNA are known.<\/p>\n\n\n\n<p>Restriction endonucleases are a special class that recognize and cleave specific sequences in DNA.&nbsp;Type II restriction endonucleases&nbsp;always cleave at or near their recognition sites. They produce small, well-defined fragments of DNA that help to characterize genes and genomes and that produce recombinant DNAs. Fragments of DNA produced by restriction endonucleases can be moved from one organism to another. In this way it has been possible to express proteins such as human insulin in bacteria.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Nucleases are enzymes that hydrolytically&nbsp;cleave&nbsp;the phosphodiester backbone of DNA.&nbsp;Endonucleases cleave in the middle of chains, while exonucleases operate selectively by degrading from the end of the chain. Nucleases that act on both single- and double-stranded DNA are known. Restriction endonucleases are a special class that recognize and cleave specific sequences in DNA.&nbsp;Type II restriction endonucleases&nbsp;always [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[],"class_list":["post-1864","post","type-post","status-publish","format-standard","hentry","category-nucleic-acids"],"Cooking_time":"","jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/cake.appscodestudio.com\/index.php\/wp-json\/wp\/v2\/posts\/1864","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cake.appscodestudio.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/cake.appscodestudio.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/cake.appscodestudio.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/cake.appscodestudio.com\/index.php\/wp-json\/wp\/v2\/comments?post=1864"}],"version-history":[{"count":2,"href":"https:\/\/cake.appscodestudio.com\/index.php\/wp-json\/wp\/v2\/posts\/1864\/revisions"}],"predecessor-version":[{"id":1939,"href":"https:\/\/cake.appscodestudio.com\/index.php\/wp-json\/wp\/v2\/posts\/1864\/revisions\/1939"}],"wp:attachment":[{"href":"https:\/\/cake.appscodestudio.com\/index.php\/wp-json\/wp\/v2\/media?parent=1864"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/cake.appscodestudio.com\/index.php\/wp-json\/wp\/v2\/categories?post=1864"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/cake.appscodestudio.com\/index.php\/wp-json\/wp\/v2\/tags?post=1864"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}