The strands of the DNA double helix are held together by\u00a0hydrogen bonding\u00a0interactions between the complementary base pairs. Heating DNA in\u00a0solution\u00a0easily breaks these hydrogen bonds, allowing the two strands to separate\u2014a process called denaturation or\u00a0melting. The two strands may reassociate when the solution cools, reforming the starting DNA duplex\u2014a process called renaturation or hybridization. These processes form the basis of many important techniques for\u00a0manipulating\u00a0DNA. For example, a short piece of DNA called an oligonucleotide can be used to test whether a very long DNA sequence has the complementary sequence of the oligonucleotide embedded within it. Using hybridization, a single-stranded DNA molecule can capture complementary sequences from any source. Single strands from RNA can also reassociate. DNA and RNA single strands can form hybrid molecules that are even more stable than double-stranded DNA. These molecules form the basis of a technique that is used to purify and characterize\u00a0messenger RNA\u00a0(mRNA) molecules corresponding to single genes.<\/p>\n","protected":false},"excerpt":{"rendered":"
Denaturation The strands of the DNA double helix are held together by\u00a0hydrogen bonding\u00a0interactions between the complementary base pairs. Heating DNA in\u00a0solution\u00a0easily breaks these hydrogen bonds, allowing the two strands to separate\u2014a process called denaturation or\u00a0melting. The two strands may reassociate when the solution cools, reforming the starting DNA duplex\u2014a process called renaturation or hybridization. These […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[],"class_list":["post-1856","post","type-post","status-publish","format-standard","hentry","category-nucleic-acids"],"Cooking_time":"","jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/cake.appscodestudio.com\/index.php\/wp-json\/wp\/v2\/posts\/1856","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cake.appscodestudio.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/cake.appscodestudio.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/cake.appscodestudio.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/cake.appscodestudio.com\/index.php\/wp-json\/wp\/v2\/comments?post=1856"}],"version-history":[{"count":1,"href":"https:\/\/cake.appscodestudio.com\/index.php\/wp-json\/wp\/v2\/posts\/1856\/revisions"}],"predecessor-version":[{"id":1857,"href":"https:\/\/cake.appscodestudio.com\/index.php\/wp-json\/wp\/v2\/posts\/1856\/revisions\/1857"}],"wp:attachment":[{"href":"https:\/\/cake.appscodestudio.com\/index.php\/wp-json\/wp\/v2\/media?parent=1856"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/cake.appscodestudio.com\/index.php\/wp-json\/wp\/v2\/categories?post=1856"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/cake.appscodestudio.com\/index.php\/wp-json\/wp\/v2\/tags?post=1856"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}