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What do proteins do?
Proteins are essential for life and are essential for a wide range of cellular activities. Protein enzymes catalyze the vast majority of chemical reactions that occur in the cell. Proteins provide many of the structural elements of a cell, and they help to bind cells together into tissues. Proteins, in the form of antibodies, protect animals from disease, and many hormones are proteins. Proteins…
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Where is protein stored?
Proteins are not stored for later use in animals. When an animal consumes excess proteins, they are converted into fats (glucose or triglycerides) and used to supply energy or build energy reserves. If an animal is not consuming sufficient protein, the body begins to break down protein-rich tissues, such as muscles, leading to muscle wasting and eventually death if…
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Where does protein synthesis take place?
Protein synthesis occurs in the ribosomes of cells. In eukaryotic cells, ribosomes are found as free-floating particles within cells and are also embedded in the rough endoplasmic reticulum, a cell organelle.
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What is a protein?
A protein is a naturally occurring, extremely complex substance that consists of amino acid residues joined by peptide bonds. Proteins are present in all living organisms and include many essential biological compounds such as enzymes, hormones, and antibodies.
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Translation
The process of translation uses the information present in the nucleotide sequence of mRNA to direct the synthesis of a specific protein for use by the cell. Translation takes place on the ribosomes—complex particles in the cell that contain RNA and protein. In prokaryotes the ribosomes are loaded onto the mRNA while transcription is still ongoing. Near the 5′…
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Transcription
Small segments of DNA are transcribed into RNA by the enzyme RNA polymerase, which achieves this copying in a strictly controlled process. The first step is to recognize a specific sequence on DNA called a promoter that signifies the start of the gene. The two strands of DNA become separated at this point, and RNA polymerase begins copying from…
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RNA metabolism
RNA provides the link between the genetic information encoded in DNA and the actual workings of the cell. Some RNA molecules such as the rRNAs and the snRNAs (described in the section Types of RNA) become part of complicated ribonucleoprotein structures with specialized roles in the cell. Others such as tRNAs play key roles in protein synthesis, while mRNAs…
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Repair
It is extremely important that the integrity of DNA be maintained in order to ensure the accurate workings of a cell over its lifetime and to make certain that genetic information is accurately passed from one generation to the next. This maintenance is achieved by repair processes that constantly monitor the DNA for lesions and activate appropriate…
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Site-specific recombination
Site-specific recombination involves very short specific sequences that are recognized by proteins. Long DNA sequences such as viral genomes, drug-resistance elements, and regulatory sequences such as the mating type locus in yeast can be inserted, removed, or inverted, having profound regulatory effects. More than any other mechanism, site-specific recombination is responsible for reshaping genomes. For…
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General recombination
General recombination, also called homologous recombination, involves two DNA molecules that have long stretches of similar base sequences. The DNA molecules are nicked to produce single strands; these subsequently invade the other duplex, where base pairing leads to a four-stranded DNA structure. The cruciform junction within this structure is called a Holliday junction, named after Robin…
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