Repair

It is extremely important that the integrity of DNA be maintained in order to ensure the accurate workings of a cell over its lifetime and to make certain that genetic information is accurately passed from one generation to the next. This maintenance is achieved by repair processes that constantly monitor the DNA for lesions and activate appropriate repair enzymes. As described in the section General recombination, serious lesions in DNA such as pyrimidine dimers or gaps can be repaired by recombination mechanisms, but there are many other repair mechanisms.

One important mechanism is that of mismatch repair, which has been studied extensively in E. coli. The system is directed by the presence of a methyl group within the sequence GATC on the template strand. Comparable systems for mismatch repair also operate in eukaryotes, though the template strand is not marked by methyl groups. In fact, lesions within the genes for human mismatch repair systems are known to be responsible for many cancers. Loss of the mismatch repair system allows mutations to build up quickly and eventually to affect the genes that cause cells to divide. As a result, cells divide in an uncontrolled manner and become cancerous.

Once replication is complete, the most common kind of damage to nucleic acids is one in which the normal A, C, G, and T bases are changed into chemically modified bases that usually differ significantly from their natural counterparts. The only exceptions are the deamination of cytosine to uracil and the deamination of 5-methylcytosine to thymine. In these cases the product is a G:U or G:T mismatch. Specific enzymes called DNA glycosylases can recognize uracil in DNA or the thymine in a G:T mismatch and can selectively remove the base by cleaving the bond between the base and the deoxyribose sugar. Many of these enzymes are specific for the different chemically modified bases that may be present in DNA.

Another common means of repairing DNA lesions is by an excision repair pathway. Enzymes recognize damage within DNA, probably by detecting an altered conformation of DNA, and then nick the strand on either side of the lesion, allowing a small single-stranded DNA to be excised. DNA polymerase and DNA ligase then repair the single-stranded gap. In all of these systems, the presence of an abnormal base signifies which strand is to be repaired, and the complementary strand is used as the template to ensure the accuracy of repair.